Publications – Center for ImmunoEngineering

Li S, Siriwon N, Zhang X, Yang S, Jin T, He F, Kim YJ, Mac J, Lu Z, Wang S, Han X, Wang P. Enhanced cancer immunotherapy by chimeric antigen receptor-modified T cells engineered to secrete checkpoint inhibitors. Clin Cancer Res, 2017, accepted.
Siegler E, Kim YJ, Chen X, Siriwon N, Mac J, Rohrs J, Bryson P, Wang P. Combination cancer therapy using chimeric antigen receptor-engineered natural killer cells as drug carriers. Mol Ther, 2017, accepted.
Siegler E, Li S, Kim YJ, Wang P. Designed ankyrin repeat proteins as Her2 targeting domains in chimeric antigen receptor-engineered T cells. Hum Gene Ther, 2017, Epub ahead of print [ Link ]
Han X, Cinay GE, Zhao Y, Guo Y, Zhang X, Wang P. Adnectin-based design of chimeric antigen receptor for T cell engineering. Mol Ther, 2017, Epub ahead of print [ Link ]
Kim JT, Liu Y, Kulkarni RP, Lee KK, Dai B, Lovely G, Ouyang Y, Wang P, Yang L, Baltimore D. Dendritic cell-targeted lentiviral vector immunization uses pseudotransduction and DNA-mediated STING and cGAS activation. Sci Immunol, 2017, 2(13). pii: eaal1329. [ Link ]
Zhang X, Liu Y, Kim YJ, Mac J, Zhuang R, Wang P. Co-delivery of carboplatin and paclitaxel via cross-linked mulipamellar liposomes for ovarian cancer treatment. RSC Adv, 2017, 7(32):19685-19693. [ Link ]
Han X, Bryson PD, Zhao Y, Cinay GE, Li S, Guo Y, Siriwon N, Wang P. Masked chimeric antigen receptor for tumor-specific activation. Mol Ther, 2017, 25(1):274-284. [ Link ]
Siegler EL, Kim YJ, Wang P. Nanomedicine targeting the tumor imcroenvironment: Therapeutic strategies to inhibit angiogenesis, remodel maxtrix, and modulate immune responses J Cellular Immunotherapy, 2016, 2(2):69-78. [ Link ]
Kim YJ, Eiegler EL, Siriwon N, Wang P. Therapeutic strategies for targeting cancer stem cells J Cancer Metasta Treat, 2016, 2:233-242. [ Link ]
Rohrs JA, Wang P, Finley SD. Predictive model of lymphocyte-specific protein tyrosine kinase (LCK) autoregulation. Cell Mol Bioeng, 2016, 9:351-367. [ Link ]
Fiacco SV, Kelderhouse LE, Hardy A, Peleg Y, Hu B, Ornelas A, Yang P, Gammon ST, Howell SM, Wang P, Takahashi TT, Millward SW, Roberts RW. Directed evolution of scanning unnatural-protease-reistant (SUPR) peptide for in vivo applications. ChemBioChem, 2016, 17(17): 1643-1651. [ Link ]
Fang J, Hu B, Li S, Zhang C, Liu Y, Wang P. A multi-antigen vaccine in combination with an immunotoxin targeting tumor-associated fibroblast for treating murine melanoma Mol Ther Oncolytics, 2016, 3:16007. [ Link ]
Fang J, Xiao L, Joo KI, Liu Y, Zhang C, Liu S, Conti PS, Li Z, Wang P. A potent immunotoxin targeting fibroblast activation protein for treatment of breast cancer in mice. Int J Caner, 2016, 138(4), 1013-1023. [ Link ]
Kim YJ, Liu Y, Li S, Rohrs J, Zhang R, Zhang X, Wang P. Co-erdication of breast cancer cells and cancer stem cells by cross-linked multilamellar liposomes enhances tumor treatment. Mol Pharm, 2015, 12(8), 2811-2822. [ Link ]
Liu Y, Siriwon N, Rohrs JA, Wang P. Generation of targeted adeno-associated virus (AAV) vectors for human gene therapy. Curr Pharm Des, 2015, 21(22), 3248-3256. [ Link ]
Zhang C, Hu B, Xiao L, Liu Y, Wang P. Pseudotyping lentiviral vectors with lymphocytic choriomeningitis virus glycoproteins for transduction of dendritic cells and in vivo immunization. Hum. Gene Ther. Methods, 2014, 25(6), 328-338. [ Link ]
Zhao M, Liu Y, Hsieh RS, Wang N, Tai W, Joo KI, Wang P, Gu Z, Tang Y. Clickable protein nanocapsules for targeted delivery of recombinant p53 protein. JACS. 2014, 136(43), 15319-15325. [ Link ]
Liu Y, Fang J, Joo KI, Wong MK., Wang P. Codelivery of chemotherapeutics via crosslinked multilamellar liposomal vesicles to overcome multidrug resistance in tumor. PLoS One. 2014, 9(10): e110611. [ Link ]
Liu Y, Xiao L, Joo KI, Hu B., Fang J, Wang P. In situ modulation of dendritic cells by injectable thermo-sensitive hydrogels for cancer vaccines in mice. Biomacromolecules. 2014, 15(10), 3836-3845. [ Link ]
Howell SM, Fiacco SV, Takahashi TT, Jalali-Yazdi F, Millward SW, Hu B, Wang P, Roberts, RW. Serum stable natural peptides designed by mRNA display. Scientific Reports. 2014, 4: 6008. [ Link ]
Liu Y, Rohrs J, Wang P. Development and challenges of nanovectors in gene therapy, Nano LIFE. 2014, 04, 1441007. [ Link ]
Liu Y, Rohrs J, Wang P. Advances and challenges of nanoparticles in optimizing PK/PD interactions of combination chemotherapies. Current Drug Metabolism. 2014, accepted.
Lee CL, Liu Y, Dang J, Joo KI, Wang P. Mutagenesis of the E1 domain of sindbis virus glycoprotein alters its pH-dependent fusion. Nano LIFE. 2014, accepted. [ Link ]
Yang L, Wang P. Passive immunization against HIV/AIDS by antibody gene transfer.Viruses. 2014, 6(2):428-47. [ Link ]
Liu Y, Fang J, Kim YJ, Wong MK, Wang P. Codelivery of doxorubicin and paclitaxel by cross-linked multilamellar liposome enables synergistic antitumor activity. Mol Pharm. 2014, 11(5):1651-61. [ Link ]
Liu Y, Kim YJ, Ji M, Fang J, Siriwon N, Zhang L, Wang P. Enhancing in vitro and in vivo gene delivery of adeno-associated virus type 2 by cell-permeable peptides.Molecular Therapy – Methods & Clinical Development, 2014, 1, 12. [ Link ]
Liu Y, Joo KI, Lei Y, Wang P. Visualization of intracellular pathways of engineered baculovirus in mammalian cells. Virus Res. 2014, 181:81-91. [ Link ]
Liu Y, Fox V, Lei, Y, Hu B, Joo KI, Wang P. Synthetic niches for directed differentiation of human embryonic stem cells bypassing embryoid body formation. J Biomed Mater Res B: Appl Biomater. 2014, 102(5):1101-12. [ Link ]
Liu Y, Tai A, Joo KI, Wang P. Visualization of DC-SIGN-mediated entry pathway of engineered lentiviral vectors in target cells. PLoS One. 2013, 15, 780-783. (doi: 10.1021/ol303435y) [ Link ]
Liu Y, Ji M, Wong MK, Joo KI, Wang P. Enhanced therapeutic efficacy of iRGD-conjugated crosslinked multilayer liposomes for drug delivery. 2013. BioMed Res. Int., 2013, 2013:378380. (doi: 10.1155/2013/378380) [ Link ]
Bryson PD, Zhang C, Lee CL, Wang P. A tetracycline-regulated cell line produces large-scale quantities of lentiviral vectors that specifically target dendritic cells. J. Vis. Exp. 2013, June 19 (76). (doi: 10.3791/50606) [ Link ]
Choo YH, Fang J, Liu H, Filler S, Wang P, Tang Y. Genome mining of a prenylated immunosuppressive polyketide from pathogenic fungi. Org. Lett., 2013, 15, 780. (doi: 10.1021/ol303435y; PMID: 23368997) [ Link ]
Xiao L, Hung KC, Takahashi TT, Joo KI, Lim M, Roberts RW, Wang P. Antibody-mimetic ligand selected by mRNA display targets DC-SIGN for dendritic cell-directed antigen delivery, 2013, 8, 967-977. (doi: 10.1021/cb300680c) [ Link ]
Joo KI, Xiao L, Liu S, Liu Y, Lee CL, Conti PS, Wong MK, Li Z, Wang P., Crosslinked multilamellar liposomes for controlled delivery of anticancer drugs, Biomaterials 2013, 34, 3098. (doi: 10.1016/j.biomaterials.2013.01.039) [ Link ]
Zhao M, Hu B, Gu Z, Joo KI, Wang P, Tang Y. Degradable polymeric nanocapsule for efficient intracellular delivery of a high molecular weight tumor-selective protein complex, Nano Today, 2013, 8, 11-20. (doi: 10.1016/j.nantod.2012.12.003) [ Link ]
Liu Y, Joo KI, Wang P. Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells, Gene Ther. 2013, 20(3):308-17. (doi: 10.1038/gt.2012.41; PMID: 22622241) [ Link ]
Liu Y, Fang Y, Zhou Y, Zandi E, Lee CL, Joo KI, Wang P. Site-specific modification of adeno-associated viruses via a genetically engineered aldehyde tag, Small, 2013, 9(3):421-9. (doi: 10.1002/smll.201201661; PMID: 23038676) [ Link ]
Xiao L, Joo KI, Lim M, Wang P. Dendritic cell-directed vaccination with a lentivector encoding PSCA for prostate cancer in mice, PLoS One, 2012, 7:e48866. (doi: 10.1371/journal.pone.0048866; PMID: 23139820) [ Link ]
Chooi YH, Wang P, Fang J, Li Y, Wu K, Wang P, Tang Y. Discovery and characterization of a group of fungal polycyclic polyketide prenyltransferases, J. Am. Chem. Soc., 2012, 134, 9428-9437. (doi: 10.1021/ja3028636; PMID: 22590971) [ Link ]
Dai B, Xia0 L, Bryson PD, Wang P. PD-1/PD-L1 blockade can enhance HIV-1 Gag-specific T cell immunity elicited by dendritic cell-directed lentiviral vaccines, Mol. Ther., 2012, 20, 1800-1809. (doi: 10.1038/mt.2012.98; PMID: 22588271) [ Link ]
Xiao L, Kim J, Lim M, Dai B, Yang L, Reed SG, Baltimore D, Wang P. A TLR4 agonist synergizes with dendritic cell-directed lentiviral vectors for inducing antigen-specific immune responses, Vaccine, 2012, 30, 2570-2581. (PMCID: PMC3360926) [ Link ]
Lee CL, Chou M, Dai B, Xiao L, Wang P. Construction of stable producer cells to make high-titer lentiviral vectors for dendritic cell-based vaccination, Biotech. Bioeng., 2012, 109, 1551-1560. (doi: 10.1002/bit.24413; PMCID: PMC3330149) [ Link ]
Zhao M, Biswas A, Hu B, Joo KI, Wang P, Gu Z, Tang Y. Redox-responsive nanocapsules for intracellular protein delivery, Biomaterials, 2011, 32, 5223-5230. (PMID: 21514660) [ Link ]
Lee CL, Dang J, Joo KI, Wang P. Engineered lentiviral vectors pseudotyped with a CD4 receptor and a fusogenic protein can target cells expressing HIV-1 envelope proteins, Virus Res., 2011, 160, 340-350. (doi: 10.1016/j.virusres.2011.07.010; PMCID: PMC3495169) [ Link ]
Froelich S, Tai A, Kennedy K, Zubair A, Wang P. Virus-receptor mediated transduction of dendritic cells by lentiviruses enveloped with glycoproteins derived from semliki forest virus, PLoS One, 2011, 6:e21491. (PMCID: PMC3124512) [ Link ]
Froelich S, Tai A, Kennedy K, Zubair A, Wang, P. Pseudotyping lentiviral vectors with aura virus envelope glycoproteins for DC-SIGN-mediated transduction of dendritic cells, Hum. Gene Ther., 2011, 22, 1281-1291. (PMCID: PMC3205794) [ Link ]
Joo KI, Fang Y, Liu Y, Xiao L, Gu Z, Tai A, Lee CL, Tang Y, Wang P. Enhanced real-time monitoring of adeno-associated virus trafficking by virus-quantum dot conjugates, ACSNano, 2011, 5, 3523-3535. (PMCID: PMC3132586) [ Link ]
Biswas A, Joo KI, Liu J, Zhao M, Fan G, Wang P, Gu Z, Tang Y. Endoprotease-mediated intracellular protein delivery using nanocapsules, ACSNano, 2011, 5, 1385-1394. (PMID: 21368592) [ Link ]
Tai A, Froelich S, Joo KI, Wang P. Production of lentiviral vectors with enhanced efficiency to target dendritic cells by attenuating mannosidase activity of mammalian cells, J. Bio. Eng., 2011, 5:1. (PMCID: PMC3039557) [ Link ]
Lei Y, Lee CL, Joo KI, Zarzar J, Liu Y, Dai B, Fox V, Wang P. Gene editing of human embryonic stem cells via an engineered baculoviral vector carrying zinc finger nucleases, Mol. Ther., 2011, 19, 942-950. (PMCID: PMC3098635) [ Link ]
Yang H, Hu B, Liang X, Wang P. Dendritic cell-directed lentivector vaccine induces antigen-specific immune responses against murine melanoma, Cancer Gene Ther., 2011, 18, 370-380. (PMID: 21372855) [ Link ]
Hu B, Tai A, Wang P. Immunization delivered by lentiviral vectors for cancer and infectious diseases, Immunol. Rev., 2011, 239, 45-61. (PMCID: PMC3030993) [ Link ]
Nagarah JM, Paek E, Luo Y, Wang P. Hwang GS, Heath JR. Batch fabrication of high-performance planar patch-clamp devices in quartz, Adv. Mater., 2010, 22, 4622-4627. [ Link ]
Hu B, Dai B, Wang P. Vaccines delivered by integration-deficient lentiviral vectors targeting dendritic cells induces strong antigen-specific immunity, Vaccine, 2010, 28, 6675-6683. (PMCID: PMC2956406) [ Link ]
Gu Z, Biswas A, Joo KI, Hu B, Wang P, Tang Y. Probing protease activity by single-fluorescent-protein nanocapsules, Chem. Commun., 2010, 46, 6467-6469. [ Link ]
Joo KI, Tai A, Lee CL, Wong C, Wang P. Imaging multiple intermediates of single-virus membrane fusion mediated by distinct fusion proteins, Microsc. Res. Tech., 2010, 73, 886-900. (PMCID: PMC2930027) [ Link ]
Lei Y, Joo KI, Zarzar J, Wong C, Wang P. Targeting lentiviral vector to specific cell types through surface displayed single chain antibody and fusogenic molecule, Virol. J., 2010, 7:35. (PMCID: PMC2830192) [ Link ]
Froelich S, Tai A, Wang P. Lentiviral vectors for immune cells targeting, Immuopharmacol. Immunotoxico., 2010, 32, 208-218. (PMCID: PMC2864798) [ Link ]
Gu Z, Yan M, Hu B, Joo KI, Biswas A, Lu Y, Wang P, Tang Y. Protein nanocapsule weaved with enzymatically degradable polymeric network, Nano Lett., 2009, 9, 4533-4538. (PMID: 19995089) [ Link ]
Dai B, Yang L, Yang H, Hu B, Baltimore D, Wang P. HIV-1 Gag-specific immunity induced by a lentivector-based vaccine directed to dendritic cells, Proc. Natl. Acad. Sci. USA, 2009, 106, 20382-20387. (PMCID: PMC2777969) [ Link ]
Hu B, Yang H, Dai B, Tai A, Wang P. Nonintegrating lentiviral vectors can effectively deliver ovalbumin antigen for induction of antitumor immunity, Hum. Gene Ther., 2009, 20, 1652-1664. (PMCID: PMC2799785) [ Link ]
Tang Y, Wang P, Van Deventer JA, Link AJ, Tirrell DA. Introduction of an aliphatic ketone into recombinant proteins in a bacterial strain that overexpresses an editing-impaired lucyl-tRNA synthetase, ChemBioChem, 2009, 10, 2188-2190. [ Link ]
Li R, Beebe T, Cui J, Rouhanizadeh M, Ai L, Wang P, Gundersen M, Takabe W, Hsiai TK. Pulsatile shear stress increased mitochondrial membrane potential: implication of Mn-SOD, Biochem. Biophys. Res. Commun., 2009, 388, 406-412. [ Link ]
Froelich S, Ziegler L, Stroup K, Wang P. Targeted gene delivery to CD117-expressing cells in vivo with lentiviral vectors co-displaying stem cell factor and a fusogenic molecule, Biotech. Bioeng., 2009, 104, 206-215. [ Link ]
Lei Y, Joo KI, Wang P. Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors, J. Biol. Eng., 2009, 3:8. [ Link ]
Yang H, Joo KI, Ziegler L, Wang P. Cell type-specific targeting with surface-engineered lentiviral vectors co-displaying OKT3 antibody and fusogenic molecule, Pharm. Res., 2009, 26, 1432-1445. [ Link ]
Dai B, Wang P. In vitro differentiation of adult bone marrow progenitors into antigen-specific CD4 helper T cells using engineered stromal cells expressing a Notch ligand and a MHC class II protein, Stem Cells Dev., 2009, 18, 325-246. [ Link ]
Luo XM, Maarschalk E, O’Connell RM, Wang P, Yang L, Baltimore D. Engineering human hematopoietic stem/progenitor cells to produce a broadly neutralizing anti-HIV antibody after in vitro maturation to human B lymphocytes. Blood, 2009 113, 1422-1431. [ Link ]
Chhabra A, Yang L, Wang P, Comin-Anduix B, Das R, Chakraborty NG, Ray S, Mehrotra S, Yang H, Hardee CL, Hollis R, Dorsky DI, Koya R, Kohn DB, Ribas A, Economou JS, Baltimore D, Mukherji B. CD4 CD25 T cells transduced to express MHC class I-restricted epitope-specific TCR synthesize Th1 cytokines and exhibit MHC class I-restricted cytolytic effector function in a human melanoma model, J. Immunol., 2008, 181, 1063-1070. [ Link ]
Joo KI, Lei Y, Lee CL, Lo J, Xie J, Hamm-Alvarez SF, Wang P. Site-specific labeling of enveloped viruses with quantum dots for single virus tracking, ACS Nano, 2008, 2, 1553-1562. [ Link ]
Joo KI, Wang P. Visualization of targeted transduction by engineered lentiviral vectors, Gene Ther., 2008, 15, 1384-1596. [ Link ]
Ziegler L, Yang L, Joo KI, Lei Y, Yang H, Baltimore D, Wang P. Targeting lentiviral vectors to antigen-specific immunoglobulins, Hum. Gene Ther., 2008, 19, 861-872. [ Link ]
Yang H, Ziegler L, Joo KI, Cho T, Lei Y, Wang P. Gamma-retroviral vectors enveloped with an antibody and an engineered fusogenic protein achieved antigen-specific targeting, Biotech. Bioeng., 2008, 101, 357-368. [ Link ]
Yang L, Yang H, Rideout K, Cho T, Joo KI, Ziegler L, Elliot A, Walls A, Yu D, Baltimore D, Wang P. Engineered lentivector targeting of dendritic cells for in vivo immunization, Nat. Biotech., 2008, 26, 326-334. [ Link ]
Yang L, Bailey L, Baltimore D, Wang P. Targeting lentiviral vectors to specific cell types in vivo, Proc. Natl. Acad. Sci. USA, 2006, 103, 11479-11484. [ Link ]
Kwon I, Wang P, Tirrell DA. Design of a bacterial host for site-specific incorporation of para-bromophenylalanine into recombinant proteins, J. Am. Chem. Soc., 2006, 128, 11778-11783.
Wang P, Fichera A, Kumar K, Tirrell DA. Alternative translations of a single RNA message: an identity switch of (2S,3R)-4,4,4-trifluorovaline between valine and isoleucine codons, Angew. Chem. Int. Ed., 2004, 43, 3664-3666.
Wang P, Tang Y, Tirrell DA. Incorporation of trifluoroisoleucine into proteins in vivo, J. Am. Chem. Soc., 2003, 125, 6900-6906.
Wang P, Vaidehi N, Tirrell DA, Goddard III WA. Virtual screening for binding of phenylalanine analogs to phenylalanyl-tRNA synthetase, J. Am. Chem. Soc., 2002, 124, 14442-14449.
Datta D, Wang P, Carrico IS, Mayo SL, Tirrell DA. A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins, J. Am. Chem. Soc., 2002, 124, 5652-5653.
Kumaraswamy G, Verma RK, Issaian AM, Wang P, Kornfield JA, Yeh F, Hsiao BS, Olley RH. Shear induced crystallization in isotactic polypropylene Part 2: analysis of the formation of the oriented ‘Skin’, Polymer, 2000, 41, 8931-8940.
Zhang Q, Sun X, Wang P. Preparation of graded index plastic rods doped with Sm3+ by interfacial-gel polymerization, Polymer International, 1998, 45, 185-190.
Zhang Q, Wang P, Zhai Y. Preparation of graded index plastic rods doped with Nd3+ by interfacial-gel polymerization, J. Appl. Polym. Sci., 1998, 67, 1431-1436.
Zhang Q, Wang P, Sun X, Zhai Y, Dai P, Yang B, Hai M, Xie J. Amplified spontaneous emission of an Nd3+-doped Poly(methyl methacrylate) optical fiber at ambient temperature, Appl. Phys. Lett., 1998, 74, 407-409.
Zhang Q, Wang P. Analysis on absorption spectra of Nd3+ in poly(methyl methacrylate), J. Mol. Stru., 1998, 440, 35-42.
Zhang Q, Wang P, Zhai Y. Refractive index distribution of graded index poly(methyl methacrylate) preform made by interfacial-gel polymerization, Macromolecules, 1997, 30, 7874-7879.